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[KO Validated] N-Cadherin Rabbit mAb

時間：2020-10-29 09:57   瀏覽次數(shù)：1365次

品牌 ABclonal 產品類型 抗體
產品名稱 [KO Validated] N-Cadherin Rabbit mAb 產品規(guī)格 50ul /100ul /200ul
產品目錄號 A19083 產品目錄價 800/1200/2000

背景

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

免疫原信息

應用

反應物種Human, Mouse, Rat
驗證應用WBIHCIF
推薦稀釋比&實驗步驟
  • WB   1:500 - 1:2000  [ 實驗步驟 ]
  • IHC   1:50 - 1:200  [ 實驗步驟 ]
  • IF   1:50 - 1:200  [ 實驗步驟 ]
理論分子量140kDa
實際分子量140kDa
存儲緩沖液Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
實驗方法Western blotting    
陽性樣本HeLa,293T,NIH/3T3,C6
細胞定位
純化方式Affinity purification

[KO Validated] N-Cadherin Rabbit mAb 檢測圖片

ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (A19083) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A19083) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry of paraffin-embedded mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry of paraffin-embedded rat heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083) }

Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunofluorescence analysis of mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.